phosphor akt (Proteintech)
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Proteintech
phosphor akt
Phosphor Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+akt/Phospho-AKT1+(Ser473)+Antibody/pm41483432-76-75-99
Average 96 stars, based on 519 article reviews
Phosphor Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+akt/Phospho-AKT1+(Ser473)+Antibody/pm41483432-76-75-99
Average 96 stars, based on 519 article reviews
phosphor akt - by Bioz Stars,
2026-10
96/100 stars
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Luminescence Assay:Article Title: Design, synthesis and biological evaluation of aminopyrimidine derivatives bearing dihydroquinoxalinone as novel EGFRL858R/T790M kinase inhibitors against non-small-cell lung cancer Article Snippet: .. Materials RPMI-1640 medium (ATCC, Rockville, MD, USA, 30-2001), MEM medium (Gibco, Invitrogen Corporation, NY, USA, 11095080), penicillin and streptomycin (Sigma, St. Louis, MO, USA), L-alany-L-glutamine (Zhongqiaoxinzhou Biotech, China, CSP004), sodium pyrurate (Sigma, USA, S9133), Kinase substrate 22 (GL, China, 112393), CCK8 (Beyotime, Shanghai, China), 96- well plates (Biosahrp, BS-MP-96W), CellTiter-Glo luminescent assay (Promega-G7573, USA), Osimertinib (MedChemExpress, Shanghai, China), FITC-conjugated Annexin V (KeyGEN Biotech, NanJing, China, KGA108), PMSF (Solon, OH, USA), NC membranes (Millipore, Schwalbach, Germany), phosphor-EGFR (CST, Tyr1068, #3777T), EGFR (AB clonal, #A11351), Incubation:Article Title: Forkhead Box q1 promotes invasion and metastasis in colorectal cancer by activating the epidermal growth factor receptor pathway. Article Snippet: Equal amounts of protein (30 μg) were resolved on a 10% sodium dodecyl sulfate (SDS)-precast polyacrylamide gel (Bio–Rad Laboratories) and Zhang JJ et al. FOXQ1 promotes CRC invasion and metastasis WJG https://www.wjgnet.com 1784 May 7, 2022 Volume 28 Issue 17 transferred to an Immobilon-polyvinylidene difluoride membrane (Millipore, Billerica, MA, United States). .. The membranes were blocked and incubated with the following primary antibodies: FOXQ1 (Abcam, Cambridge, MA, United States), HB-EGF (Abcam, Cambridge, MA, United States), phosphorPI3K (Cell Signaling Technology, Cold Spring Harbor, NY, United States), PI3K (Proteintech, Wuhan, China), Akt (Proteintech, Wuhan, China), Article Title: Design, synthesis and biological evaluation of aminopyrimidine derivatives bearing dihydroquinoxalinone as novel EGFRL858R/T790M kinase inhibitors against non-small-cell lung cancer Article Snippet: .. After blocking in 5% non-fat dried milk in TBS, membranes were incubated with primary antibodies against phosphor-EGFR (CST, Tyr1068, #3777T), EGFR (AB clonal, #A11351), Article Title: Exploration of novel dihydroquinoxalinone derivatives as EGFR L858R/T790M tyrosine kinase inhibitors for the treatment of non-small-cell lung cancer. Article Snippet: To overcome or delay the drug-resistance of first-generation epidermal growth factor receptor (EGFR) kinase inhibitors and non-selectivity toxicity mediated by second-generation inhibitors, splicing principle was employed to design and synthesize a series of Osimertinib derivatives containing dihydroquinoxalinone (8–30) as the novel third-generation inhibitors against double mutant L858R/T790M in EGFR.. Among them, compound 29 showed excellent kinase inhibitory activity against EGFRL858R/T790M with an IC50 value of 0.55 ± 0.02 nM and potent anti-proliferative activity against H1975 cells with an IC50 value of 5.88 ± 0.07 nM.. Moreover, the strong downregulation effect of EGFR-mediated signaling pathways and the promotion of apoptosis in H1975 cells confirmed its potent antitumor activities. Article Title: Design, synthesis and biological evaluation of aminopyrimidine derivatives bearing dihydroquinoxalinone as novel EGFRL858R/T790M kinase inhibitors against non-small-cell lung cancer Article Snippet: After the protein concentrations were determined by a BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, Illinois, USA), the protein extracts were reconstituted in loading buffer and boiled at 100 °C for 10 min. An equal amount of the protein (20 μg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to NC membranes (Millipore, Schwalbach, Germany). .. After blocked with 5% non-fat dried milk in TBS containing 1% Tween-20 for 60 min at room temperature, the membrane was incubated overnight with primary antibodies against phosphor-EGFR (CST, Tyr1068, #3777T), EGFR (AB clonal, #A11351), Article Title: Network pharmacology integrated with experimental validation to explore the therapeutic role and potential mechanism of Epimedium for spinal cord injury Article Snippet: .. Next, the PVDF papers were blocked using skim milk for 1.5 h, then incubated overnight with the primary antibodies against GAPDH (1: 1000, Goodhere), Article Title: Repression of Polycomb Group Factor 1 Inhibits EMT Progression in Colon Cancers Cells via Wnt/β-Catenin and PI3K/Akt/mTOR Signaling Pathway. Article Snippet: .. Skim milk (5%) was used to seal the membrane at room temperature for 1 h, and the primary antibody was diluted at 1:1000 and incubated overnight at 4°C (PCGF1, ab259943, Abcam, RRID: AB_10672187; β-actin, ab8827, Abcam, RRID: AB_2305186; N-cadherin, 22,018–1-AP, proteintech, RRID: AB_2813891; E-cadherin, 20,874–1-AP, proteintech, RRID: AB_10697811; Snail-1, 13,099–1-AP, proteintech, RRID: AB_2191756; mTOR, 2972, Cell Signaling Technology, RRID: AB_330978; phosphor-mTOR [Ser2448], 2971, Cell Signaling Technology, RRID: AB_330970; Akt, 9272, Cell Signaling Technology, RRID: AB_329827; Blocking Assay:Article Title: Design, synthesis and biological evaluation of aminopyrimidine derivatives bearing dihydroquinoxalinone as novel EGFRL858R/T790M kinase inhibitors against non-small-cell lung cancer Article Snippet: .. After blocking in 5% non-fat dried milk in TBS, membranes were incubated with primary antibodies against phosphor-EGFR (CST, Tyr1068, #3777T), EGFR (AB clonal, #A11351), Article Title: Exploration of novel dihydroquinoxalinone derivatives as EGFR L858R/T790M tyrosine kinase inhibitors for the treatment of non-small-cell lung cancer. Article Snippet: To overcome or delay the drug-resistance of first-generation epidermal growth factor receptor (EGFR) kinase inhibitors and non-selectivity toxicity mediated by second-generation inhibitors, splicing principle was employed to design and synthesize a series of Osimertinib derivatives containing dihydroquinoxalinone (8–30) as the novel third-generation inhibitors against double mutant L858R/T790M in EGFR.. Among them, compound 29 showed excellent kinase inhibitory activity against EGFRL858R/T790M with an IC50 value of 0.55 ± 0.02 nM and potent anti-proliferative activity against H1975 cells with an IC50 value of 5.88 ± 0.07 nM.. Moreover, the strong downregulation effect of EGFR-mediated signaling pathways and the promotion of apoptosis in H1975 cells confirmed its potent antitumor activities. Membrane:Article Title: Design, synthesis and biological evaluation of aminopyrimidine derivatives bearing dihydroquinoxalinone as novel EGFRL858R/T790M kinase inhibitors against non-small-cell lung cancer Article Snippet: After the protein concentrations were determined by a BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, Illinois, USA), the protein extracts were reconstituted in loading buffer and boiled at 100 °C for 10 min. An equal amount of the protein (20 μg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to NC membranes (Millipore, Schwalbach, Germany). .. After blocked with 5% non-fat dried milk in TBS containing 1% Tween-20 for 60 min at room temperature, the membrane was incubated overnight with primary antibodies against phosphor-EGFR (CST, Tyr1068, #3777T), EGFR (AB clonal, #A11351), Article Title: Repression of Polycomb Group Factor 1 Inhibits EMT Progression in Colon Cancers Cells via Wnt/β-Catenin and PI3K/Akt/mTOR Signaling Pathway. Article Snippet: .. Skim milk (5%) was used to seal the membrane at room temperature for 1 h, and the primary antibody was diluted at 1:1000 and incubated overnight at 4°C (PCGF1, ab259943, Abcam, RRID: AB_10672187; β-actin, ab8827, Abcam, RRID: AB_2305186; N-cadherin, 22,018–1-AP, proteintech, RRID: AB_2813891; E-cadherin, 20,874–1-AP, proteintech, RRID: AB_10697811; Snail-1, 13,099–1-AP, proteintech, RRID: AB_2191756; mTOR, 2972, Cell Signaling Technology, RRID: AB_330978; phosphor-mTOR [Ser2448], 2971, Cell Signaling Technology, RRID: AB_330970; Akt, 9272, Cell Signaling Technology, RRID: AB_329827; |
